Human SHP1 also known as, HCP, HCPH, SHP-1, HPTP1C, PTP-1C, SHP-1L, SH-PTP1, and PTPN6 GenBank Accession No. NM_002831, a.a. 2-595 (full length) with N-terminal His-tag, expressed in an E. coli cell expression system. MW =68 kDa.
Specific activity: 6.6 pmole/min/ug
Immunogen Region
2-595 (full length)
Tag
N-terminal His-tag
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween20, 20% glycerol, 3 mM DTT, and 22 0mM Imidazole.
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
Enzyme reaction (50 ul) containing 20 mM HEPES, pH 7.4, 100 mM NaCl, 2 mM EDTA, 2 mM DTT, 1 mM pNPP and 0-8000 ng SHP-1. Activity monitored at 415 nm continuously with readings at 1-minute intervals for 20 minutes.