Human recombinant SHP-1, also known as tyrosine-protein phosphatase non-receptor type 6 (PTPN6), protein tyrosine phosphatase 1 C (PTP-1C), and SH-PTP1, Genbank accession number NM_002831, a.a. 221-523 with N-terminal His-tag. MW=36 kDa, expressed in an E. coli expression system.
Immunogen Region
221-523
Tag
N-terminal His-Tag
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween20, 20% glycerol, 3 mM DTT, 220mM Imidazole.
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
Enzyme reaction (50 ul) containing 20 mM HEPES, pH 7.2, 100 mM NaCl, 2 mM EDTA, 10 mM DTT, 1 mM pNPP and PTPase was monitored at 415 nm continuously with readings at 1 minute intervals for 20 minutes.
Avoid freeze/thaw cycles.
GenBank
NM_002831
Mw(kda)
36 kDa
Synonyms
tyrosine-protein phosphatase non-receptor type 6 (PTPN6), protein tyrosine phosphatase 1 C (PTP- 1C), SH-PTP1, shp1
Uniprot
P29350
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Stebbins, C.C., et al., Mol. Cell Biol. 2003; 23: 6291-6299.2. Ganesan, L.P., et al., J. Biol. Chem. 2003; 278: 35710-35717.