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Home / Proteins & Peptide

Shh (C24IVI), Human

SKU : BHP18500244

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Mfr.No.	IX03069, IX03069-1, IX03069-10, IX03069-50
Description	Sonic Hedgehog (Shh) is a member of the Hedgehog (Hh) family of highly conserved proteins which are widely represented throughout the animal kingdom. In mammal, there are three related Hh proteins, Sonic (Shh), Desert (Dhh) and Indian (Ihh). They share a high degree of amino-acid sequence identity (e.g., Shh and Ihh are 93% identical). Sonic Hedgehog plays a role in cell growth, cell specialization, and the normal shaping (patterning) of the body. Shh is also important for development of the brain and spinal cord (central nervous system), eyes, limbs, and many other parts of the body.Recombinant human Sonic Hedgehog (C24IVI) (rhShh) produced in E. coli is a single non-glycosylated polypeptide chain containing 176 amino acids. A fully biologically active molecule, rhShh has a molecular mass of 19.8 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques.
Reconstitution	Reconstituted in ddH2O at 100 ug/ml.
Formulation	Lyophilized after extensive dialysis against PBS.
Molecular Weight	19.8 kDa, observed by reducing SDS-PAGE.
Source	E. coli
Host	Human
Accession Number	Q15465
Endotoxin Level	0.2 EU/ug, determined by LAL method.
Notes	For research use only
Bioactivity	ED50 2.0 ug/ml, measured by its ability to induce alkaline phosphatase production by C3H/10T1/2 (CCL-226) Cells, corresponding to a specific activity of 500 units/mg.

Synonyms	Hhg1
Sequence	IVIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGRYEGKISRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQRCKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHYEGRAVDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHIHCSVKAENSV AAKSGG

Purity	95% by SDS-PAGE analysis.
Storage	Lyophilized recombinant human Sonic Hedgehog (C24IVI) (rhShh) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rhShh should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHP18500244-1	IX03069-1	1mg	$1498		Add to Cart
BHP18500244-2	IX03069-10	10ug	$82		Add to Cart
BHP18500244-3	IX03069-50	50ug	$199		Add to Cart

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