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Home / Proteins & Peptide

Set8, Active, GST-tag

SKU : BHP18200408

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Mfr.No.	51008
Description	Human SET8 (GenBank Accession No. NM_020382), a.a. 195-352 with N-terminal GST-tag, MW = 44 kDa, expressed in an E. coli expression system.
Background	SETD8 or SET domain containing lysine methyltransferase 8 is a lysine methyltransferase that monomethylates p53 in human cell lines. The monomethylation by SETD8 suppresses p53-mediated transcriptional activation of highly responsive target genes, such as p21 (CDKN1A) and PUMA (BBC3) but it has little influence on weak p53 targets. Depletion of SET8 augmented the proapoptotic and checkpoint activation functions of p53, and SETD8 expression is downregulated during DNA damage. SETD8 is a Wnt signaling mediator and is recruited by LEF1/TCF4 to regulate the transcription of Wnt-activated genes, possibly through H4K20 monomethylation at the target gene promoters.
Immunogen Region	195-352
Tag	N-terminal GST-tag
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathione
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	50 ul reaction mix (50 mM Tris, pH 8.8, 1 mM EDTA, 1 mM DTT, 40 uM S-adenosylhomocysteine, and 0-6 ug SET8) is added to the wells coated with the substrate. Incubate for 2 hr. Add antibody against methylated residue of histone H4, incubate 1 hr. Then, add secondary HRP-labeled antibody and incubate 30 min. Finally, add HRP chemiluminsecent substrates and read luminescence. Avoid freeze/thaw cycles.
GenBank	NM_020382
Mw(kda)	44 kDa

Synonyms	PR-Set7, SET07, SET8, SETD8
Uniprot	Q9NQR1

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Fang,J. et al., Curr. Biol. 12 (13), 1086-1099 (2002). 2. Yin,Y. et al., Cell Cycle 7 (10), 1423-1432 (2008).

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200408	51008	50 ug	$357.50		Add to Cart

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