Human SAP145, a substrate for PRMT9, also known as Splicing Factor 3b Subunit 2 (SF3B2), (GenBank accession No. NM_006842), (a.a. 401-550) with an N-terminal GST-tag, MW= 45kDa, expressed in a Baculovirus-infected Sf9 cell expression system.
Immunogen Region
401-550
Tag
N-terminal GST-tag
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween20, 16mM glutathione, 20% glycerol
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended. If necessary, use carrier protein (BSA 0.1 - 0.5%).
GenBank
NM_006842
Mw(kda)
45 kDa
Synonyms
SAP145, Splicing Factor 3b Subunit 2, SF3B2m, SAP
Uniprot
Q13435
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Yang, Y., et al. (2015) Nat Commun 6, 64282. Falk, S., et al (2016) Nat Commun 7, 13573