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Home / Proteins & Peptide

ROS1, GST-tag

SKU : BHP18200211

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BPS Bioscience

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Mfr.No.	40268
Description	Human Proto-oncogene c-Ros-1 (ROS1), also known as MCF3 (GenBank Accession No. NM_002944), a.a. 1883-2347(end) with N-terminal GST-tag, MW= 78 kDa, expressed in Sf9 cells via a baculovirus expression system. Host cell line: Sf9 cells Specific activity: 8 pmol/min/ug
Immunogen Region	1883-2347(end)
Tag	N-terminal GST-tag
Formulation	25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 20% glycerol, and 3 mM DTT.
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Assayed using Z-Lyte (Invitrogen) kinase assay conditions with 2 uM Tyr 01 Peptide in 50 mM HEPES, pH 7.5, 0.01% BRIJ-35, 10 MgCl₂, 1 mM EGTA for 45 minutesat 30°C, and incubated with reagent B (1:256) for 50 minutes at RT. Results were read using a Tecan Infinite M1000. Avoid freeze/thaw cycles.
GenBank	NM_002944
Mw(kda)	65 kDa

Synonyms	ROS, c-Ros Oncogene 1, MCF3
Uniprot	P08922

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Charest, A., et al. (2003). Proc. Natl. Acad. Sci. USA 100 (3): 916-21. 2. Sharma, S., et al. (1990). Oncogene Res. 5 (2): 91-100. 3. Keilhack, H., et al. (2001). J. Cell Biol. 152 (2): 325-34.

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200211	40268	10 ug	$401.50		Add to Cart

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