Human ring finger protein 20, or RNF20 (GenBank Accession No. NM_019592), full length, a.a. 2-975 (end) with N-terminal His-FLAG-tag, MW=115 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Immunogen Region
2-975 (end)
Tag
N-terminal His-FLAG-tags
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 80 ng/ul Flag peptide, 20% glycerol and 3 mM DTT.
Species
Human
Application
Useful in conjunction with E1 and E2 for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Notes
50 ul reaction mix (4 mM ATP, 5 mM MgCl2, 1 mM DTT, E1 (0.5 ug), E2 (2.8 ug), Biotin-Ubiquitin and RNF20 in 50 mM Tris (pH 7.4) incubate for 30 min at 37°C. Then add to streptavidin coated plates and incubate for 30 min at 37°C. Add antibody against RNF20, incubate 1 hour. Finally, incubate 1 hour with the HRP-labeled secondary antibody.