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Home / Proteins & Peptide

RIPK2, GST-tag

SKU : BHP18200180

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BPS Bioscience

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Mfr.No.	40173
Description	Recombinant human RIPK2(GenBank Accession No. NM_003821), (a.a. 1-299) with N-terminal Gst-tag,MW=59 kDa, expressed in an baculovirusinfected Sf9 cell expression system. Host cell line: Sf9 cells Specific activity: 20 pmol/min/ug
Background	RIPK2 (RIP2; RICK) is a death domain-containing protein kinase encoding a predicted 540-amino acid protein which contains an N-terminal serine/threonine kinase catalytic domain and a C-terminal caspase activation and recruitment domain. RIPK2 is thought to regulate apoptosis induced by the FAS receptor pathway (1). RIPK2 has been shown to specifically interact with the CARD of ICE/caspase-1 and this interaction correlates with the processing of pro-caspase-1 and the formation of the active caspase-1 p20 (2).
Immunogen Region	1-299
Tag	N-terminal GST-tag
Formulation	50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol.
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Assay Buffer: 25mM MOPS, pH 7.2, 12.5mM beta-glycerophophate, 25mM MgCl2, 5mM EGTA, 2mM EDTA.Incubate RIPK2 with substrate (0.2mg/ml) and (³²P)-ATP at 30°C for 15 minutes, then spot reaction on P81 paper, fix in 1% phosphoric acid, and assay with a scintillation counter. Avoid freeze/thaw cycles.
GenBank	NM_003821
Mw(kda)	59 kDa

Synonyms	RICK; RIP2; CARD3; CARDIAK; CCK; GIG30
Uniprot	O43353

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Purity	>=75%
Storage	At least 6 months at -80°C.

Reference	1. Inohara, N. et al: RICK, a novel protein kinase containing a caspase recruitment domain, interacts with CLARP and regulates CD95-mediated apoptosis. J. Biol. Chem. 273: 12296-12300, 1998. Note: Erratum: J. Biol. Chem 273: 18675 only, 1998. 2. Thome, M. et al: Identification of CARDIAK, a RIP-like kinase that associates with caspase-1. Curr. Biol. 8: 885-888, 1998.

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200180	40173	10 ug	$429		Add to Cart

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