Human Ret (GenBank Accession No. NM_020975), a.a. 658-end with N-terminal GST-tag, MW= 77.5 kDa, expressed in an Baculovirus infected Sf9 cell expression system.
Host cell line: Sf9 cells
Specific activity: 52 pmol/min/ug.
Unit definition: One unit is defined as the amount of enzyme that will transfer 1 pmol phosphate to Tyr substrate per minute at pH 7.4 and 30°C.
Immunogen Region
658-end
Tag
N-terminal GST-tag
Formulation
25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, 10 mM reduced glutathione, and 3 mM DTT.
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 uM Na orthovanadate, 0.1 mM ATP, 30 ug/ml Poly (Glu:Tyr)4:1 substrate, and 2 ug/ml recombinant Ret.
Avoid freeze/thaw cycles.
GenBank
NM_020975
Mw(kda)
77.5 kDa
Synonyms
RET, CDHF, CDHR, Cadherin-Related Family Member 16, Hydroxyaryl-Protein Kinase, MEN2A, Cadherin Family Member 12
Uniprot
P07949
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Moore,S.W., Appfelstaedt,J. and Zaahl,M.G. J. Pediatr. Surg. 42 (2), 326-332 (2007). 2 Griseri,P., Lantieri,F., Puppo,F., Bachetti,T., Di Duca,M., Ravazzolo,R. and Ceccherini,I. Hum. Mutat. 28 (2), 168-176 (2007).