A DNA sequence encoding the the Zika virus (strain Zika SPH2015) NS1 (ALU33341.1) (Val796-Leu1157) was expressed with a polyhistidine tag at the C-terminus.
Alternative Names
Zika virus, NS1 protein, strain Zika SPH2015
Reconstitution
Please refer to the printed manual for detailed information.
Background
Zika virus NS1 antigen is one of seven non-structural proteins. NS1 is involved in RNA replicaiton. The possible effects of NS1 on hosts include: localization to host cell surface and secreted extracellularly, modelates signalling of innate immune system, has possible damages to platelets and endothelial cells through anti-NS1 antibodies.
Reactivity
ZIKV
Tag
C-His
Formulation
Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 8.0, 10 % gly.Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual.
Host
Baculovirus-Insect Cells
Accession Number
ALU33341.1
Endotoxin Level
< 1.0 EU per ?g protein as determined by the LAL method.
Mol Weight
42.6kDa
Mw(kda)
42.6kDa
Sequence Info
Val796-Leu1157
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped with ice bag. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stotage Note
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.