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Recombinant ZIKV (strain Zika SPH2015) Envelope protein (Domain III, His Tag)

SKU : BHP10306040

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Elabscience Biotechnology Co.,Ltd

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Mfr.No.	PKSV030271
Description	A DNA sequence encoding the Zika virus (strain Zika SPH2015) E / Envelope (domain III) (ALU33341.1) (Val593-Lys699) was expressed with a polyhistidine tag at the C-terminus.
Alternative Names	Zika virus, E protein, strain Zika SPH2015
Reconstitution	Please refer to the printed manual for detailed information.
Background	Envelope of Zika virus is resposible for receptor binding and membrane. Analysis of the envelope protein of Zika, from Brazilian Zika SPH215 (KU321639), indicates predicted B and T cell epitopes in peptides that are consistent to those reported for dengue, YFYF and Japanese encephalitis. The envelope Domain II B cell epitope, to which much dengue non-neutralizing cross reaction is attributed, is also conserved also in Zika virus, consistent with prior field observations of cross reactivity with dengue and YF. Domain III of the Zika envelope protein, likely the main specific neutralizing domain, is distinct from recent Brazilian dengue isolates and a recent Peruvian YF isolate (GQ379163), 76% of possible major histocompatibility complex class (MHC) I and MHC II binding peptides and potential B cell linear epitopes are unique to Zika virus.
Reactivity	ZIKV
Tag	C-His
Formulation	Lyophilized from sterile PBS, pH 7.4.Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual.
Host	HEK293 Cells
Accession Number	ALU33341.1
Endotoxin Level	< 1.0 EU per ?g protein as determined by the LAL method.
Mol Weight	13kDa.
Mw(kda)	13kDa.

Sequence Info	Val593-Lys699

Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped with ice bag. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stotage Note	Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Purity	> 90 % as determined by SDS-PAGE.

Product Manual	Data Sheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP10306040	PKSV030271	100ug	$855		Add to Cart

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