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Home / Proteins & Peptide

Recombinant ZIKV E / Envelope protein (Domain I, His & MBP Tag)

SKU : BHP10306033

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Elabscience Biotechnology Co.,Ltd

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Mfr.No.	PKSV030264
Description	A DNA sequence encoding the Zika virus (strain Zika SPH2015) E (ALU33341.1) (Ile291-Thr399 and Glu426-Gly485 and Ser576- Gly592) was expressed with a N-terminal polyhistidine-tagged MBP tag at the N-terminus (his-MBP).
Alternative Names	Zika virus, E protein, strain Zika SPH2015
Reconstitution	Please refer to the printed manual for detailed information.
Background	Envelope of Zika virus is resposible for receptor binding and membrane. Analysis of the envelope protein of Zika, from Brazilian Zika SPH215 (KU321639), indicates predicted B and T cell epitopes in peptides that are consistent to those reported for dengue, YFYF and Japanese encephalitis. The envelope Domain II B cell epitope, to which much dengue non-neutralizing cross reaction is attributed, is also conserved also in Zika virus, consistent with prior field observations of cross reactivity with dengue and YF. Domain III of the Zika envelope protein, likely the main specific neutralizing domain, is distinct from recent Brazilian dengue isolates and a recent Peruvian YF isolate (GQ379163), 76% of possible major histocompatibility complex class (MHC) I and MHC II binding peptides and potential B cell linear epitopes are unique to Zika virus.
Reactivity	ZIKV
Tag	N-His&MBP
Formulation	Lyophilized from sterile 250 mM NaCl, 50 mM Tris, pH 8.0.Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual.
Host	E. coli
Accession Number	ALU33341.1
Endotoxin Level	Please contact us for more information.
Mol Weight	57.2kDa
Mw(kda)	57.2kDa

Sequence Info	Ile291-Thr399 and Glu426-Gly485 and Ser576- Gly592

Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped with ice bag. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stotage Note	Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Purity	> 75 % as determined by SDS-PAGE.

Product Manual	Data Sheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP10306033	PKSV030264	100ug	$855		Add to Cart

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