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Home / Proteins & Peptide

Recombinant TNF-α, Mouse (P. pastoris-expressed)

SKU : BHP11300305

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Mfr.No.	BK0348‐100μg
Description	Sharing 79% sequence identity with human Tumor Necrosis Factor-alpha (hTNF-α), mouse Tumor Necrosis Factor-alpha (mTNF-α) is a cytokine mainly expressed by immune cells [1]. A type II transmembrane protein, TNF-α is further proteolytically processed to a soluble form [2]. The trimeric active TNF-α[3] then exerts its diverse biological properties including gapoptosis, inflammation, autoimmunity and cell proliferation by binding to TNF Receptor 1 and 2 [4]. Recombinant mouse Tumor Necrosis Factor-alpha (rmTNF-α) produced in P.pastoris is a fully biologically active polypeptide chain of 152 amino acids with a molecular mass of 17 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
Alternative Names	TNF-alpha ; TNF-α ; Tumornecrosis factor ligand super family member 2 ; TNF-a ; TNFa ; Cachectin ; DIF ; TNFA ; TNFSF 2
Regulatory	RUO
Source	P. pastoris
Host	Mouse
Endotoxin Level	<1 EU/μg, determined by LAL method.
Biological Activity Comment	ED50<0.01ng/ml, measured by cytotoxicity assay using L929 cells, corresponding to a specific activity of >1 x 10ˆ8units/mg.
Nature	Recombinant

Weight	17kDa, observed by reducing SDS-PAGE.

Description	Reconstituted in ddH2O or PBS at 100 μg/ml.
Format	Lyophilized after extensive dialysis against PBS. Sterile Filtered White lyophilized (freeze-dried) powder.
Purity	> 95% as analyzed by reducing SDS-PAGE.
Storage	Lyophilized recombinant mouse Tumor Necrosis Factor-alpha (rmTNF-α) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rmTNF-αshould be stable up to 1 week at 4°C or up to 2 months at -20°C.

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SKU	Supplier No.	Size	Your price	Quantity
BHP11300305	BK0348‐100μg	100μg	$485.20		Add to Cart
BHP11300305	BK0348‐100μg	1mg	$2055.56		Add to Cart
BHP11300305	BK0348‐100μg	20μg	$188.67		Add to Cart

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