Tumor Necrosis Factor-Alpha (TNF-alpha) plays a major role in growth regulation, differentiation, inflammation, viral replication, tumorigenesis, and autoimmune diseases. Besides inducing hemorrhagic necrosis of tumors, TNF has been found to be involved in tumorigenesis, tumor metastasis, viral replication, septic shock, fever, inflammation, and autoimmune diseases including Crohn's disease, and rheumatoid arthritis as well as graft-versus-host disease. TNF alpha-1a is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells and certain other target cells.Recombinant mouse Tumor Necrosis Factor-alpha (rmTNF-alpha) produced in E. coli is a non-glycosylated polypeptide chain of 163 amino acids. A fully biologically active molecule, rhTNF-alpha has a molecular mass of 18.3kDa analyzed by reducing SDS-PAGE and is obtained by proprietary refolding and chromatographic techniques at GenScript.
Alternative Names
TNF-alpha ; TNF-α ; Tumornecrosis factor ligand super family member 2 ; TNF-a ; TNFa ; Cachectin ; DIF ; TNFA ; TNFSF 2
Tag
His
Regulatory
RUO
Source
Escherichia coli.
Host
Mouse
Endotoxin Level
<1 EU/μg, determined by LAL method.
Biological Activity Comment
ED50<0.05ng/ml, measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D, corresponding to a specific activity of > 2x 10ˆ7units/mg.
Nature
Recombinant
Weight
18.3kDa, observed by reducing SDS-PAGE.
Description
Reconstituted in ddH2O at 100 μg/ml.
Format
Lyophilized after extensive dialysis against PBS.Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
> 95% as analyzed by reducing SDS-PAGE.
Storage
Lyophilized recombinant mouse Tumor Necrosis Factor-alpha (rmTNF-slpha) remains stable up to 12 months at -80°C from date of receipt. Upon reconstitution, rmTNF-alpha should be stable up to 4 week at 4°C or up to 6 months at -20°C.