Recombinant Taq Plus DNA Polymerase

Mfr.No.	enz-309
Description	Taq Plus is a mixture of Taq and Pfu. Taq Plus, which is used to improve the reliability and yield of conventional primer extension reaction. Taq Plus has two following advantages over Taq: (1) high fidelity with an error frequency 1.6/106 (or 0.0016/103) during DNA synthesis. (2) Taq Plus increases the efficiency of polymerization reaction, resulting in a great percentage of extenuation reaction completion up to 10 kb to 30 kb. Pfu has a temperature optimum between 72-78°C and remains > 95% active following 1-hour incubation at 95°C.
Alternative Names	Taq Plus DNA Polymerase ; Taq Plus DNA ; TaqPDNA
Regulatory	RUO
Source	Recombinant E.coli contains Thermus aquaticus polymerase gene
Nature	Recombinant

Shipping	Shipped with Ice Packs
Buffer	Storage Buffer: 20mM Tris-HCl (pH 8.0), 1mM DTT, 0.1mM EDTA, 100mM KCl, Stabilizers, and 50%glycerol. 10X Reaction Buffer: 200 mM TrisHCI (pH 8.8)100 mM KCI100 mM (NH4)2SO420 mM Mg SO41% Triton X-1001 mg/ml bovine serum albumin (BSA).
Description	Formulation: 5U/µl. Unit Definition: One unit of the enzyme catalyzes the incorporation of 10nmole of deoxyribonucleotides into a polynucleotide fraction in 30 min at 74°C. Optimization of DNA Synthesis: It is important to ad the reaction components in the following order1. H2O.2. 10 x reaction buffer.3. dNTPs.4. DNA template and primers.5. Taq Plus. Reaction Conditions: DNA synthesis is performed in 100?l of mixture containing 20-200μm dNTPs, 0.3-1μm Promers, 0.1-0.250 mg of template DNA, 10 ?l of 10 x reaction buffer and 2.5-5 units of Taq Plus. Mix the reaction gently, centrifuge briefly and then overlay with light mineral oil. Initially, denature the reaction by incubating at 95? for 5 minutes and then cool to 40-68? for 5 minutes to allow the primers to anneal to the template DNA. Note: All reagents, including Taq Plus, should be mixed immediately before use.
Expiry Date	Stable for 5 days at 10°C, for longer period of time store at -20°C.

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SKU	Supplier No.	Size	Your price
BHP11001101	enz-309	250U	$60	Add to Cart
BHP11001101	enz-309	750U	$145	Add to Cart
BHP11001101	enz-309	2	$60	Add to Cart
BHP11001101	enz-309		$350	Add to Cart