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Home / Proteins & Peptide

Recombinant Taq DNA Polymerase

SKU : BHP11001100

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ProSpec-Tany TechnoGene Ltd.

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Mfr.No.	enz-308
Description	Taq DNA Polymerase(a) is a thermostable enzyme of approximately 95 kDa isolated from Thermus aquaticus. This unmodified enzyme replicates DNA at 74°C and exhibits a half-life of 40 minutes at 95°C. The enzyme catalyzes the polymerization of nucleotides into duplex DNA in the 5´~3´ direction in the presence of magnesium and also possesses a 5´~3´ exonuclease activity. Taq DNA Polymerase is recommended for use in PCR but is not recommended for use in DNA sequencing reactions.
Alternative Names	DNA polymerase I thermostable ; EC 2.7.7.7 ; Taq polymerase 1
Regulatory	RUO
Source	Recombinant e.coli contains Thermus aquaticus polymerase gene
Nature	Recombinant

Uniprot	P19821

Shipping	Shipped with Ice Packs
Buffer	Storage Buffer: Compatibility with Reaction Buffers: Taq DNA Polymerase in Storage Buffer. Use of other reaction buffers that do not contain Triton X-100 (final concentration of 0.1%) will result in inactivation of the enzyme. 50mM Tris-HCl (pH 8.0), 100mM NaCl, 0.1mM EDTA, 1mM DTT, 50% glycerol and 1% Triton X-100. 10X Reaction Buffer with MgCl2: 500mM KCl, 100mM Tris-HCl (pH 9.0 at 25C), 1% Triton X-100 and 15mM MgCl2. Buffer is optimized for use with 0.2mM of each dNTP. 10X Reaction Buffer without MgCl2: 500mM KCl, 100mM Tris-HCl (pH 9.0 at 25C) and 1% Triton X-100. Include: 10X Reaction Buffer without MgCl2 and separate 25mM MgCl2 Solution.
Description	Formulation: Taq DNA Polymerase solution in 20mM Tris-HCl, pH 8.0, 100mM KCl, 0.1mM EDTA, 1mM DTT, 50% Glycerol, 0.5% NP40, 0.5% Tween 20. Unit Definition: One unit is defined as the amount of enzyme required to catalyze the incorporation of 10nmol of dNTP into acid-insoluble material in 30 minutes at 74°C. The reaction conditions are: 50mM Tris-HCl (pH 9.0 at 25°C), 50mM NaCl, 5mM MgCl2, 200µm each of dATP, dCTP, dGTP, dTTP (a mix of unlabeled and [3H]dTTP), 10µg activated calf thymus DNA and 0.1mg/ml BSA in a final volume of 50ul. Rely on a Performance-Tested Enzyme: Our PCR systems, enzymes and reagents are proven in PCR to ensure reliable, high-performance results. If you are not completely satisfied with any of our PCR product, we will send a replacement or refund your account. Specify Your Own Reaction Conditions: Choose either Taq with Mg-free 10X Reaction Buffer and separate 25mM MgCl2 or Taq with 10X Reaction Buffer containing 15mM MgCl2. Note: Depend on an Enzyme That Works: Compositions of the storage buffers have been optimized to assure quality performance of the enzyme under a variety of conditions.
Purity	Greater than 95.0% as determined by SDS-PAGE.
Expiry Date	Stable for 5 days at 10°C, for longer period of time store at -20°C.

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SKU	Supplier No.	Size	Your price	Quantity
BHP11001100	enz-308	1	$60		Add to Cart
BHP11001100	enz-308		$60		Add to Cart
BHP11001100	enz-308	3	$60		Add to Cart
BHP11001100	enz-308		$145		Add to Cart
BHP11001100	enz-308	10	$60		Add to Cart
BHP11001100	enz-308		$350		Add to Cart

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