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Home / Proteins & Peptide

Recombinant Shh, Mouse

SKU : BHP11300140

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Mfr.No.	BK0162‐10μg
Alternative Names	SHH ; HHG-1
Antigen	SHH
Description	Sonic Hedgehog (Shh) is a member of the Hedgehog (Hh) family of highly conserved proteins which are widely represented throughout the animal kingdom. In mammal, there are threerelatedHh proteins, Sonic (Shh), Desert (Dhh) and Indian (Ihh). They share a high degree of amino-acid sequence identity (e.g., Shh and Ihh are 93% identical). Sonic Hedgehog plays a role in cell growth, cell specialization, and the normal shaping (patterning) of the body. Shh is also important for development of the brain and spinal cord (central nervous system), eyes, limbs, and many other parts of the body.Recombinant mouseSonic Hedgehog(rmShh) produced in E.coli is a single non-glycosylated polypeptide chain containing 176 amino acids. A fully biologically active molecule, rmShh,is obtained by proprietary chromatographic techniques at GenScript with a molecular mass of 19.8kDa analyzed by reducing SDS-PAGE.
Regulatory	RUO
Source	Escherichia coli.
Host	Mouse
Endotoxin Level	<0.2 EU/μg, determined by LAL method.
Biological Activity Comment	ED50<1.0μg/ml, measured by its ability to induce alkaline phosphatase production by C3H/10T1/2 (CCL-226) Cells, corresponding to a specific activity of>1.0× 10ˆ3units/mg.
Nature	Recombinant

Weight	19.8kDa, observed by reducing SDS-PAGE.

Description	Reconstituted in ddH2O at 100 μg/ml.
Format	Lyophilized after extensive dialysis against PBS. Sterile Filtered White lyophilized (freeze-dried) powder.
Purity	> 95% by SDS-PAGE and HPLC analyses.
Storage	Lyophilized recombinant mouseSonic Hedgehog(rmShh)remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rmShhshould be stable up to 2 weeks at 4°C or up to 3 months at -20°C.

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SKU	Supplier No.	Size	Your price	Quantity
BHP11300140	BK0162‐10μg	10μg	$173.16		Add to Cart
BHP11300140	BK0162‐10μg	1mg	$2055.56		Add to Cart
BHP11300140	BK0162‐10μg	50μg	$290		Add to Cart

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