Please refer to the printed manual for detailed information.
Background
Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. The coronavirus N protein is required for coronavirus RNA synthesis and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is the most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to the formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of the N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
Tag
N-His
Formulation
Lyophilized from sterile 50mM PB, 500mM NaCl, pH 7.4.
Species
SARS-CoV-2
Host
E.coli
Accession Number
YP_009724397.2
Endotoxin Level
Please contact us for more information.
Accurate Molecular Mass(kd)
46.31 kDa
Protein Description
A DNA sequence encoding the SARS-CoV-2 Nucleocapsid (with mutations P13L, ERS31-33 deletion, R203K, G204R) (Met1-Ala419) was expressed with a polyhistidine tag at the N-terminus.
Synonyms
2019-nCoV coronavirus NP Protein; SARS-Cov-2 np Protein; Omicron Nucleoprotein Protein
This product is provided as lyophilized powder which is shipped with ice packs.
Purity
> 95 % as determined by SDS-PAGE.
Storage
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at< -20°C for 3 months.
Immunological Activity
Testing in progress
Reviews of Recombinant SARS-CoV-2 Nucleocapsid Protein (N-His Tag)(Omicron)