A DNA sequence encoding the mature form of rat TNFalpha (P16599) (Leu 80-Leu 235) was expressed and purified, with an initial Met at the N-terminus.
Alternative Names
Tnf;MGC124630;RATTNF;TNF-alpha;Tnfa
Reconstitution
Please refer to the printed manual for detailed information.
Background
Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD120a; p55/60) and TNF-R2 (TNF receptor type 2; CD120b; p75/80), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.
Reactivity
Rat
Formulation
Lyophilized from sterile PBS, pH 7.4
Host
E. coli
Accession Number
P16599
Endotoxin Level
Please contact us for more information.
Bioactivity
Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of metabolic inhibitor actinomycin D. The ED50 for this effect is typically 20-80 pg/ml.
Mol Weight
17.4 kDa
Mw(kda)
17.4 kDa
Sequence Info
Leu 80-Leu 235
Shipping
This product is provided as lyophilized powder which is shipped with ice packs.
Stotage Note
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.