Recombinant Rat Pyruvate kinase PKM(Pkm)

Mfr.No.	CSB-EP018072RA
Description	Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation
Alternative Names	Pyruvate kinase muscle isozyme Pkm2, Pykm
Tag	N-terminal 10xHis-tagged and C-terminal Myc-tagged
Regulatory	RUO
Relevance	Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation
Source	E.coli
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Protein Name	Pyruvate kinase muscle isozyme; Pkm2, Pykm
Expression Region	1-531aa
Mol Weight	62.8 kDa
Nature	Recombinant
Protein Description	Full Length

Subcellular Location	Cytoplasm, Nucleus
Uniprot	P11980, P11980
Database Links	KEGG:rno:25630 STRING:10116.ENSRNOP00000015331 UniGene:Rn.1556
Function	Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation (By similarity).
Pathway	Glucagon signaling pathway
Protein Families	Pyruvate kinase family
Research Area	Signal Transduction
Weight	62.8 kDa

Stotage Note	Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Buffer	Tris-based buffer，50% glycerol
Purity	Greater than 85% as determined by SDS-PAGE.
Storage	The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Storage Buffer	Tris-based buffer,50% glycerol