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Home / Proteins & Peptide

Recombinant Rat CLPS/Colipase Protein (His Tag)

SKU : BHP10305700

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Elabscience Biotechnology Co.,Ltd

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Mfr.No.	PKSR030146
Description	A DNA sequence encoding the rat Clps (NP_037271.1) (Met1-Gln112) was expressed with a polyhistidine tag at the C-terminus.
Alternative Names	CLPS;Colipase
Reconstitution	Please refer to the printed manual for detailed information.
Background	Colipase belongs to the colipase family. Structural studies of the complex and of colipase alone have revealed the functionality of its architecture. It is a small protein with five conserved disulphide bonds. Structural analogies have been recognised between a developmental protein, the pancreatic lipase C-terminal domain, the N-terminal domains of lipoxygenases and the C-terminal domain of alpha-toxin. Colipase can only be detected in pancreatic acinar cells, suggesting regulation of expression by tissue-specific elements. Colipase allows lipase to anchor noncovalently to the surface of lipid micelles, counteracting the destabilizing influence of intestinal bile salts. Without colipase the enzyme is washed off by bile salts, which have an inhibitory effect on the lipase. Colipase is a cofactor needed by pancreatic lipase for efficient dietary lipid hydrolysis. It binds to the C-terminal, non-catalytic domain of lipase, thereby stabilising as active conformation and considerably increasing the overall hydrophobic binding site.
Reactivity	Rat
Tag	C-His
Formulation	Lyophilized from sterile PBS, pH 7.4
Host	HEK293 Cells
Accession Number	NP_037271.1
Endotoxin Level	< 1.0 EU per ?g protein as determined by the LAL method.
Mol Weight	11.9 kDa
Mw(kda)	11.9 kDa

Sequence Info	Met1-Gln112

Shipping	This product is provided as lyophilized powder which is shipped with ice packs.
Stotage Note	Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Purity	> 95 % as determined by SDS-PAGE

Product Manual	Data Sheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP10305700	PKSR030146	100ug	$732		Add to Cart

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