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Home / Proteins & Peptide

Recombinant Pfu DNA Polymerase

SKU : BHP11001099

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ProSpec-Tany TechnoGene Ltd.

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Mfr.No.	enz-265
Description	Pfu DNA Polymerase is a thermo-stable enzyme having a Mw of about 90kDa. Pfu DNA Polymerase is derived from E. coli that and cloned from Pyrococcus furiosus strain Vc1 DSM3638. Pfu DNA Polymerase replicates DNA at 75°C, catalyzing the polymerization of nucleotides into duplex DNA in the 5´ to 3´ direction in the existence of magnesium. Pfu DNA Polymerase possesses 3´ to 5´ exonuclease (proofreading) activity. Base misinsertions that take place during polymerization are swiftly removed by the proofreading activity of the polymerase. Therefore, Pfu DNA Polymerase is suggested for use in PCR and primer extension reactions that require high-fidelity synthesis. Pfu DNA Polymerase-generated PCR fragments are blunt-ended.
Alternative Names	DNA polymerase ; EC 2.7.7.7 ; Pfu polymerase ; Pfu-DNA Polymerase
Regulatory	RUO
Source	Escherichia Coli
Nature	Recombinant

Uniprot	P61875

Buffer	10X Buffer with MgSO4: 200mM Tris-HCl (pH 8.8 at 25°C), 100mM (NH4)2SO4, 100mM KCl, 1% Triton X-100, 1mg/ml BSA, 20mM MgSO4.
Description	Formulation: 50mM Tris-HCl, pH 8.2, 1mM DTT, 0.1mM EDTA, 0.05% CHAPS and 50% glycerol. Unit Definition: 1U of enzyme catalyzes the incorporation of 10nmol of dNTP into acid-insoluble product in 30 minutes at 75°C. PCR Protocol: Add the following components to amplify 1kb DNA template: 0.2µl Pfu-DNA Polymerase.4µl 2.5mM dNTPs.5µl 10x buffer with MgSO4. 1µl Primers mix (10µM each).1µl Template.38µl ddH2O. Amplify using the following cycling parameters: Heat Soak: 1 cycle at 94°C/4 min.Denaturation: 30 cycles at 94°C/30 sec.Annealing: 30 cycles at 55°C /30 sec.Extension: 30 cycles at 72°C /90 sec. Final: 1 cycle at 72°C /5 min.
Format	Sterile liquid formulation.

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SKU	Supplier No.	Size	Your price	Quantity
BHP11001099	enz-265	100U	$145		Add to Cart
BHP11001099	enz-265	500U	$255		Add to Cart
BHP11001099	enz-265	2500U	$1000		Add to Cart

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