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Home / Proteins & Peptide

Recombinant Nucleosome (E. coli-derived), Biotin-labeled, His-tag

SKU : BHP18201819

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Mfr.No.	52048
Description	Human recombinant nucleosomes consisting of 147 bp DNA (PCR) and 2 molecules each of histones H2A (a.a. 2-130(end)), H2B (a.a. 2-126(end)), H3 (a.a. 2-137(end)), and H4 (a.a. 2-103(end)), expressed in an E. coli expression system. GenBank Accession Nos. are NM_033445, NM_003528, NM_003532, and NM_003548, respectively. Each histone protein has an N-terminal His-tag. Each H2B has a C-terminal Cys added as a biotinylation site. Total histone MW (before biotinylation) =113.8 kDa. Host cell line: E. coli
Background	Chromatin, the material into which genomic DNA is packaged in eukaryotes, is a very dynamic structure. The smallest subunit of chromatin is the nucleosome, consisting of ~147 base pairs of DNA wrapped around an octamer of core histone proteins. The histone octamer is composed of a central heterotetramer of histones H3 and H4, flanked by two heterodimers of histones H2A and H2B. Each nucleosome is separated by 10 to 60 bp of linker DNA. The resulting nucleosomal array constitutes a chromatin fiber of about 10 nm in diameter. This arrangement is folded into more condensed fibers (about 30 nm) that are stabilized by binding of a linker histone (Histone H1) to each nucleosome core. Such 30 nm fibers are then condensedin vivo to form thicker interphase fibers or the most highly compacted metaphase chromosome structures
Tag	N-terminal His-tag, Biotin
Formulation	20 mM Tris-HCl, pH 7.5, 1 mM EDTA, 1 mM DTT, and 20% glycerol.
Species	Human
Application	Substrate for histone methyltransferase or acetyltransferase assays. Ideal for screening small molecular inhibitors of histone methyltransferases and acetyltransferases for drug discovery and HTS applications. Since these nucleosomes were prepared in E
Notes	The concentration of this product is determined by Bradford and represents only the protein concentration. Avoid freeze/thaw cycles.
GenBank	NM_033445, NM_003528, NM_003532, NM_003549
Mw(kda)	113.8 kDa

Uniprot	Q7L7L0, Q16778, P68431, P62805

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	Stable at -80°C for at least 6 months from date of receipt, when stored as directed

Reference	1. Meagher, R.B., Mussar, K.J., Epigenetics Chromatin 2012 Jul 20,5(1):11. 2. Huang, H., et al., Biosystems 2012 Aug,109(2):214-9.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18201819	52048	20 ug	$621.50		Add to Cart

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