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Home / Proteins & Peptide

Recombinant Nucleosome (E. coli-derived)

SKU : BHP18200457

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BPS Bioscience

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Mfr.No.	52038
Description	Human recombinant nucleosomesconsisting of 147 bp DNA (PCR) and 2 moleculeseach of histones H2A (a.a. 2-130(end)), H2B(a.a. 2-126(end)), H3 (a.a. 2-137(end)), and H4(a.a. 2-103(end)), expressed in an E. coliexpression system. GenBank Accession Nos. areNM_033445, NM_003528, NM_003532, andNM_003548, respectively. Each histone proteinhas an N-terminal His-tag. Total histone MW =113.8 kDa. Host cell line: E. coli
Immunogen Region	H2A 2-130(end), H2B 2-126(end), H3 2-137(end), H4 2-103(end)
Formulation	20 mM Tris pH 7.5, 1 mMEDTA, 1 mM DTT and 20% glycerol
Species	Human
Application	Substrate for histone methyltransferase assays. Ideal for screening small molecular inhibitors of histone methyltransferases for drug discovery and HTS applications.
Notes	The concentration of this product is determined by Bradford and represents only the protein concentration. Avoid freeze/thaw cycles.
GenBank	NM_033445, NM_003528, NM_003532, and NM_003548
Mw(kda)	113.8 kDa

Uniprot	Q7L7L0, Q16778, P68431, P62805

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Meagher, R.B., Mussar, K.J., Epigenetics Chromatin 2012 Jul 20;5(1):11. 2. Huang, H., et al., Biosystems 2012 Aug;109(2):214-9.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200457	52038	100 ug	$555.50		Add to Cart

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