Recombinant human nucleosome consisting of 147 bp DNA (PCR) and 2 molecules each of histones H2A, encompassing amino acids 2-130(end), H2B encompassing amino acids 2-126(end), H3 encompassing amino acids 2-137(end), and H4 encompassing amino acids 2-103(end). Each histone construct contains an N-terminal His-tag (6xHis). The recombinant complex was affinity purified.
host species: E. coli
Host species: E. coli
Host cell line: E. coli
Formulation
20 mM Tris, pH 7.5, 1 mM EDTA, 1 mM DTT, and 20% glycerol
Species
Human
Application
Substrate for histone methyltransferase assays. Ideal for screening small molecular inhibitors of histone methyltransferases for drug discovery and HTS applications.
Notes
Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles. The concentration of this product is determined by Bradford and represents only the protein concentration. Avoid freeze/thaw cycles.