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Home / Proteins & Peptide

Recombinant Nucleosome (E. coli-derived)

SKU : BHP18201512

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BPS Bioscience

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Mfr.No.	52038-1
Description	Recombinant human nucleosome consisting of 147 bp DNA (PCR) and 2 molecules each of histones H2A, encompassing amino acids 2-130(end), H2B encompassing amino acids 2-126(end), H3 encompassing amino acids 2-137(end), and H4 encompassing amino acids 2-103(end). Each histone construct contains an N-terminal His-tag (6xHis). The recombinant complex was affinity purified. host species: E. coli Host species: E. coli Host cell line: E. coli
Immunogen Region	Histone H2A: 2-130(end); Histone H2B: 2-126(end); Histone H3: 2-136(end); Histone H4: 2-103(end)
Tag	N-terminal His-tag
Formulation	20 mM Tris, pH 7.5, 1 mM EDTA, 1 mM DTT, and 20% glycerol
Species	Human
Application	Substrate for histone methyltransferase assays. Ideal for screening small molecular inhibitors of histone methyltransferases for drug discovery and HTS applications.
Notes	Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles. The concentration of this product is determined by Bradford and represents only the protein concentration. Avoid freeze/thaw cycles.
GenBank	Histone H2A: NM_033445; Histone H2B: NM_003528; Histone H3: NM_003532; and Histone 4: NM_003548
Mw(kda)	113.8 kDa; Histone H2A: 15 kDa; Histone H2B: 15 kDa; Histone H3: 16 kDa; Histone H4: 12 kDa

Uniprot	Histone H2A: Q7L7L0; Histone H2B: Q16778; Histone H3: P68431; Histone H4: P62805

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Purity	>=90%
Storage	At least 6 months at -80°C.

Reference	1. Meagher, R.B., Mussar, K.J., Epigenetics Chromatin 2012 Jul 20;5(1):11. 2. Huang, H., et al., Biosystems 2012 Aug;109(2):214-9.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18201512	52038-1	100 ug	$555.50		Add to Cart

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