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Home / Proteins & Peptide

Recombinant Murine Tumor Necrosis Factor-alpha (rMuTNF-α)

SKU : BHP11300483

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Mfr.No.	PR2036
Description	Tumor necrosis factor alpha (TNF-α) is produced by neutrophils, activated lymphocytes, macrophages, NK cells, LAK cells, astrocytes endothelial cells, smooth muscle cells and some transformed cells. Mouse TNF-α occurs as a membrane-anchored form. The naturally-occurring form of TNF-α is glycosylated, but non-glycosylated recombinant TNF-α has comparable biological activity. The biologically active native form of TNF-α is reportedly a trimer. Human and murine TNF-α show approximately 79% homology at the amino acid level and crossreactivity between the two species.
Regulatory	RUO
Source	Escherichia coli.
Host	Murine
Endotoxin Level	Less than 1EU/mg of rMuTNF-α as determined by LAL method.
Biological Activity Comment	Fully biologically active when compared to standard. The ED50 as determined by the cytolysis of murine L929 cells in the presence of actinomycin D is < 0.1 ng/ml, corresponding to a specific activity of > 1×107 units/mg.
Nature	Recombinant

Weight	Approximately 17.3 kDa. The recombinant murine TNF-alpha is a soluble 157 amino acid protein which corresponds to C-terminal extracellular domain of the full length transmembrane protein.

Description	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20°C. Further dilutions should be made in appropriate buffered solutions.
Format	Lyophilized from a 0.2mm filtered solution in PBS, pH 7.2. Sterile Filtered White lyophilized (freeze-dried) powder.
Purity	>97% by SDS-PAGE and HPLC analyses.
Storage	This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.

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SKU	Supplier No.	Size	Your price	Quantity
BHP11300483	PR2036	5μg	$205.56		Add to Cart
BHP11300483	PR2036	20μg	$383.33		Add to Cart

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