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Home / Proteins & Peptide

Recombinant Murine Interleukin-1 beta (rMuIL-1β )

SKU : BHP11300387

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Mfr.No.	PR2020
Antigen	Interleukin-1 Beta
Description	IL-1β is a proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1α and IL-1β binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1β is a secreted cytokine, IL-1α is predominantly a cell-associated cytokine.
Regulatory	RUO
Source	Escherichia coli.
Host	Murine
Endotoxin Level	Less than 1EU/mg of rmIL-1β as determined by LAL method.
Biological Activity Comment	The ED50 as determined by the dose-dependent stimulation of murine D10S cells is < 0.002 ng/ml, corresponding to a specific activity of > 5 x 108 units/mg.
Nature	Recombinant

Weight	Approximately 17.5kDa, a single non-glycosylated polypeptide chain containing 153 amino acids.

Description	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20°C. Further dilutions should be made in appropriate buffered solutions.
Format	Lyophilized from a 0.2mm filtered solution in PBS, pH 7.4. Sterile Filtered White lyophilized (freeze-dried) powder.
Purity	>98% by SDS-PAGE and HPLC analyses.
Storage	This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.

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SKU	Supplier No.	Size	Your price	Quantity
BHP11300387	PR2020	2μg	$205.56		Add to Cart
BHP11300387	PR2020	10μg	$383.33		Add to Cart

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