MCP-5 (CCL12) is a chemotactic factor that attracts eosinophils, monocytes, and lymphocytes but not neutrophils. It is also a potent active monocyte chemokine that signals through CCR2. It is Involved in allergic inflammation and the host response to pathogens. Also, it may play a pivotal role during early stages of allergic lung inflammation.
<1.0 EU/μg of recombinant protein as determined by the LAL method.
Biological Activity Comment
Determined by its ability to chemoattract human monocytes using a concentration range of 20-40 ng/mL.
Weight
10.0 kDa
Description
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Format
Lyophilized PowderRecombinant MCP-5 was lyophilized from a 0.2 μm filtered 20 mM PB solution pH 7.5, 100 mM NaCl.
Purity
>95% as determined by SDS-PAGE
Storage
The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.