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Home / Proteins & Peptide

Recombinant Mouse GSK3B Protein (His Tag)(Active)

SKU : BHP10305203

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Elabscience Biotechnology Co.,Ltd

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Mfr.No.	PKSM040931
Description	A DNA sequence encoding the mouse GSK3B (Q9WV60) (Met 1-Thr 420) was fused with a polyhistidine tag at the N-terminus.
Alternative Names	7330414F15Rik;8430431H08Rik;C86142;GSK-3;GSK-3beta;GSK3
Reconstitution	Please refer to the printed manual for detailed information.
Background	GSK3B is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It Contains 1 protein kinase domain, and is expressed in testis, thymus, prostate and ovary and weakly expressed in lung, brain and kidney. GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in GSK3B gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. GSK3B participates in the Wnt signaling pathway. It is implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. GSK3B also plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization. GSK3B phosphorylates MACF1 and this phosphorylation inhibits the binding of MACF1 to microtubules which is critical for its role in bulge stem cell migration and skin wound repair. It may be required for early embryo development and neuron differentiation.
Reactivity	Mouse
Tag	N-His
Formulation	Supplied as sterile 20mM Tris, 500mM NaCl, 25% glycerol, 0.2mM DTT, pH 7.4
Host	Baculovirus-Insect Cells
Accession Number	Q9WV60
Endotoxin Level	< 1.0 EU per ?g of the protein as determined by the LAL method.
Bioactivity	1. The specific activity was determined to be 61 nmol/min/mg using synthetic Phospho-Glycogen Synthase Peptide-2 (YRRAAVPPSPSLSRHSSPHQpSEDEEE) as substrate.2. Immobilized His-mGSK3B at 10 ug/ml (100 ul/well) can bind biotinylated human HG3C-CTNNB1, EC50 of biotinylated human HG3C-CTNNB1 is 0.15-0.35 ug/ml.
Mol Weight	49 kDa
Mw(kda)	49 kDa

Sequence Info	Met 1-Thr 420

Shipping	This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt, store it immediately at<-20°C.
Stotage Note	Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Purity	> 85 % as determined by SDS-PAGE

Product Manual	Data Sheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP10305203	PKSM040931	50ug	$717		Add to Cart

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