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Home / Proteins & Peptide

Recombinant Mouse GM-CSF (CSF2)

SKU : BHP10901029

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Applied Biological Materials (abm) Inc.

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Mfr.No.	Z200079
Description	GM-CSF is a monomeric cytokine with two glycosylation sites that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. CSF2 is secreted together with other factors by T cells and macrophages following cell activation by antigens or mitogens. The signalling ‘GM-CSF:receptor’ complex is a dodecamer of two hexamers of two alpha, two beta, and two ligand subunits. While non-glycosylated and glycosylated GM-CSF show similar activities in vitro, fully glycosylated GM-CSF is biologically more active in vivo. Recombinant Mouse CSF2 is a non-glycosylated, monomeric protein.
Alternative Names	Granulocyte-macrophage colony-stimulating factor ; CSF2 ; GMCSF
Quantity	1.0 mg
Regulatory	RUO
Source	E. coli
Host	Mouse
Endotoxin Level	<1.0 EU/μg of recombinant protein as determined by the LAL method
Biological Activity Comment	The ED50 as determined by the dose-dependent stimulation BaF3 cells was < 0.1 ng/mL

Weight	14.0 kDa

Description	A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Format	Lyophilized Powder Lyophilized from 0.2 μm filtered solution in Tris, pH 7.5 and NaCl
Purity	>95% as determined by SDS-PAGE
Storage	The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Immunogen Species	Mus Musculus (Mouse)

MSDS	Growth Factors, Cytokines & Kinases MSDS
Other	Growth Factor and Cytokine Brochure

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SKU	Supplier No.	Size	Your price	Quantity
BHP10901029	Z200079	1.0mg	$3815		Add to Cart

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