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Home / Proteins & Peptide

Recombinant Mouse FGF-2/FGFb Protein (aa 1-154)(Active)

SKU : BHP10305279

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Elabscience Biotechnology Co.,Ltd

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Mfr.No.	PKSM041021
Description	Recombinant Mouse Fibroblast growth factor 2/Fibroblast Growth Factor Basic is produced by our E.coli expression system and the target gene encoding Met1-Ser154 is expressed.
Alternative Names	Fibroblast Growth Factor 2; FGF-2; Basic Fibroblast Growth Factor; bFGF; Heparin-Binding Growth Factor 2; HBGF-2; Fgf2; Fgf-2
Reconstitution	Please refer to the printed manual for detailed information.
Background	FGF basic is one of 22 mitogenic proteins of the FGF family, which show 35-60% amino acid conservation. Unlike other FGFs, FGF acidic and basic lack signal peptides and are secreted by an alternate pathway. The 17 kDa mouse sequence has 98% aa identity with rat, and 95% identity with human, bovine, and sheep FGF basic. Binding of FGF to heparin or cell surface HSPG is necessary for binding, dimerization and activation of tyrosine kinase FGF receptors. FGF basic binds other proteins, polysaccharides and lipids with lower affinity. Expression of FGF basic is nearly ubiquitous but disruption of the mouse FGF basic gene gives a relatively mild phenotype, suggesting compensation by other FGF family members. FGF basic modulates such normal processes as angiogenesis, wound healing and tissue repair, embryonic development and differentiation, neuronal function and neural degeneration. Transgenic overexpression of FGF basic results in excessive proliferation and angiogenesis is reminiscent of a variety of pathological conditions.
Reactivity	Mouse
Formulation	Lyophilized from a 0.2 um filtered solution of 20mM PB, 400mM NaCl, pH 7.0.
Host	E.coli
Accession Number	P15655
Endotoxin Level	< 1.0 EU per ?g as determined by the LAL method.
Bioactivity	Measured in a cell proliferation assay using BALB/c 3T3 cells. The ED50 for this effect is 0.3-1.8 ng/ml.
Mol Weight	17.2 kDa
Mw(kda)	17.2 kDa

Sequence Info	Met1-Ser154

Shipping	This product is provided as lyophilized powder which is shipped with ice packs.
Stotage Note	Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Purity	> 95 % as determined by SDS-PAGE

Product Manual	Data Sheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP10305279	PKSM041021	10ug	$62		Add to Cart
BHP10305279	PKSM041021	50ug	-		Add to Cart

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