A DNA sequence encoding the mature form of mouse ENPEP (P16406) (Arg41-Pro945) was expressed, with a polyhistidine tag at the N-terminus.
Alternative Names
6030431M22Rik;APA;Bp-1/6C3;ENPEP;Ly-51;Ly51
Reconstitution
Please refer to the printed manual for detailed information.
Background
ENPEP, also known as aminopeptidase A, is a member of the peptidase M1 family. Members of this family are involved in response to cadmium ion and proteolysis. They located in 6 components and are expressed in 26 plant structures. ENPEP is expressed by epithelial cells of the proximal tubule cells and the glomerulus of the nephron. It also can be detected in a variety of other tissues. ENPEP probably plays a role in regulating growth and differentiation of early B-lineage cells. It also may play a role in the catabolic pathway of the renin-angiotensin system. ENPEP is a zinc-dependent membrane-bound aminopeptidase that catalyzes the cleavage of glutamatic and aspartatic amino acid residues from the N-terminus of polypeptides. It degrades vasoconstricting angiotensin II into angiotensin III and therefore helps to regulate blood pressure.
Reactivity
Mouse
Tag
N-His
Formulation
Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4
Host
Baculovirus-Insect Cells
Accession Number
P16406
Endotoxin Level
< 1.0 EU per ?g of the protein as determined by the LAL method.
Bioactivity
Measured by its ability to cleave the fluorogenic peptide substrate, Glu-7-amido-4-methyl coumarin.The specific activity is > 200 pmoles/min/ug.
Mol Weight
106 kDa
Mw(kda)
106 kDa
Sequence Info
Arg41-Pro945
Shipping
This product is provided as lyophilized powder which is shipped with ice packs.
Stotage Note
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.