CCL25 is constitutively expressed in epithelium and mucosal vessels in the small bowel, where it interacts with gut-homing B and T cells expressing its receptor, CCR9.
<1.0 EU/μg of recombinant protein as determined by the LAL method.
Biological Activity Comment
Activity was determined by the ability to induce chemotaxis of purified human monocytes at concentrations ranging between 2-10 ng/mL.
Weight
14.0 kDa
Description
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Format
Lyophilized PowderRecombinant CCL25 was was lyophilized from 0.2 μm filtered PBS, pH 7.4.
Purity
>95% as determined by SDS-PAGE
Storage
The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity.