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Home / Proteins & Peptide

Recombinant Human USP5/ISOT Protein (His Tag)

SKU : BHP10302813

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Elabscience Biotechnology Co.,Ltd

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Mfr.No.	PKSH030782
Description	A DNA sequence encoding the human USP5 isoform short (P45974-2) (Met 1-Ser 835) was fused with a polyhistidine tag at the C-terminus.
Alternative Names	ISOT
Reconstitution	Please refer to the printed manual for detailed information.
Background	Ubiquitin carboxyl-terminal hydrolase 5, also known as Deubiquitinating enzyme 5, Isopeptidase T, Ubiquitin thiolesterase 5, Ubiquitin-specific-processing protease 5, ISOT and USP5, is a member of the peptidase C19 family. USP5 contains 2 UBA domains and one UBP-type zinc finger. The UBP-type zinc finger domain interacts selectively with an unmodified C-terminus of the proximal ubiquitin. Both UBA domains are involved in polyubiquitin recognition. The UBP-type zinc finger domain crystallizes as a dimer linked by a disulfide bond between the Cys-195 residues of both molecules, but there is no evidence that the full-length USP5 exists as a dimer. USP5 cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. USP5 is involved in unanchored 'Lys-48'-linked polyubiquitin disassembly. It binds linear and 'Lys-63'-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition.
Reactivity	Human
Tag	C-His
Formulation	Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 7.4
Host	Baculovirus-Insect Cells
Accession Number	P45974-2
Endotoxin Level	< 1.0 EU per ug as determined by the LAL method.
Mol Weight	94.7 kDa
Mw(kda)	94.7 kDa

Sequence Info	Met 1-Ser 835

Shipping	This product is provided as lyophilized powder which is shipped with ice packs.
Stotage Note	Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Purity	> 92 % as determined by reducing SDS-PAGE.

Product Manual	Data Sheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP10302813	PKSH030782	100ug	$670		Add to Cart

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