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Home / Proteins & Peptide

Recombinant Human Tumor Necrosis Factor-alpha,His (rHuTNF-α-His)

SKU : BHP11300325

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Mfr.No.	PR1126
Description	Tumor necrosis factor alpha (TNF-α), also called cachectin, is produced by neutrophils, activated lymphocytes, macrophages, NK cells, LAK cells, astrocytes endothelial cells, smooth muscle cells and some transformed cells. TNF-α occurs as a secreted, soluble form and as a membrane-anchored form, both of which are biologically active. The naturally-occurring form of TNF-α is glycosylated, but non-glycosylated recombinant TNF-α has comparable biological activity. The biologically active native form of TNF-α is reportedly a trimer. Human and murine TNF-α show approximately 79% homology at the amino acid level and crossreactivity between the two species. Two types of receptors for TNF-α have been described and virtually all cell types studied show the presence of one or both of these receptor types.
Tag	His
Regulatory	RUO
Source	Escherichia coli.
Host	Human
Endotoxin Level	Less than 1EU/mg of rHuTNF-α-his as determined by LAL method.
Biological Activity Comment	Fully biologically active when compared to standard. The Specific Activity is ≥2.0 × 107 IU/mg as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D.
Nature	Recombinant

Weight	Approximately 17.5 kDa. a single, non-glycosylated, polypeptide chain containing 157 amino acids fragment (77-233) and having a molecular mass of 21.85 kDa with an amino-terminal hexahistidine tag.

Description	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 10mM HAc to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20°C. Further dilutions should be made in appropriate buffered solutions.
Format	Lyophilized from a 0.2mm filtered concentrated solution in PBS, pH 7.0. Sterile Filtered White lyophilized (freeze-dried) powder.
Purity	>95% by SDS-PAGE and HPLC analyses.
Storage	This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.

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SKU	Supplier No.	Size	Your price	Quantity
BHP11300325	PR1126	10μg	$205.56		Add to Cart
BHP11300325	PR1126	50μg	$383.33		Add to Cart

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