Recombinant Human TNF receptor-associated factor 6(TRAF6)

Mfr.No.	CSB-EP024154HU
Alternative Names	E3 ubiquitin-protein ligase TRAF6Interleukin-1 signal transducerRING finger protein 85
Antigen	TRAF6
Tag	N-terminal 6xHis-SUMO-tagged
Regulatory	RUO
Relevance	E3 ubiquitin ligase that, together with UBE2N and UBE2V1, mediates the synthesis of 'Lys-63'-linked-polyubiquitin chains conjugated to proteins, such as IKBKG, IRAK1, AKT1 and AKT2. Also mediates ubiquitination of free/unanchored polyubiquitin chain that leads to MAP3K7 activation. Leads to the activation of NF-kappa-B and JUN. May be essential for the formation of functional osteoclasts. Ses to also play a role in dendritic cells (DCs) maturation and/or activation. Represses c-Myb-mediated transactivation, in B-lymphocytes. Adapter protein that ses to play a role in signal transduction initiated via TNF receptor, IL-1 receptor and IL-17 receptor. Regulates osteoclast differentiation by mediating the activation of adapter protein complex 1 (AP-1) and NF-kappa-B, in response to RANK-L stimulation. Together with MAP3K8, mediates CD40 signals that activate ERK in B-cells and macrophages, and thus may play a role in the regulation of immunoglobulin production
Source	E.coli
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Expression Region	1-522aa
Mol Weight	75.6kDa
Nature	recombinant
Protein Description	Full Length

Subcellular Location	Cytoplasm, Cytoplasm, cell cortex, Nucleus, Lipid droplet
Uniprot	Q9Y4K3, Q9Y4K3
Database Links	HGNC:12036 OMIM:602355 KEGG:hsa:7189 STRING:9606.ENSP00000337853 UniGene:Hs.444172
Function	E3 ubiquitin ligase that, together with UBE2N and UBE2V1, mediates the synthesis of 'Lys-63'-linked-polyubiquitin chains conjugated to proteins, such as IKBKG, IRAK1, AKT1 and AKT2. Also mediates ubiquitination of free/unanchored polyubiquitin chain that leads to MAP3K7 activation. Leads to the activation of NF-kappa-B and JUN. May be essential for the formation of functional osteoclasts. Seems to also play a role in dendritic cells (DCs) maturation and/or activation. Represses c-Myb-mediated transactivation, in B-lymphocytes. Adapter protein that seems to play a role in signal transduction initiated via TNF receptor, IL-1 receptor and IL-17 receptor. Regulates osteoclast differentiation by mediating the activation of adapter protein complex 1 (AP-1) and NF-kappa-B, in response to RANK-L stimulation. Together with MAP3K8, mediates CD40 signals that activate ERK in B-cells and macrophages, and thus may play a role in the regulation of immunoglobulin production.
Pathway	MAPK signaling pathway; NF-kappa B signaling pathway; Autophagy; Ubiquitin mediated proteolysis; IL-17 signaling pathway; NOD-like receptor signaling pathway; Osteoclast differentiation; Toll-like receptor signaling pathway; Endocytosis; Neurotrophin signaling pathway
Protein Families	TNF receptor-associated factor family, A subfamily
Research Area	Developmental Biology
Tissue Specificity	Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
Weight	75.6kDa

Stotage Note	Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Buffer	Tris-based buffer，50% glycerol
Purity	Greater than 90% as determined by SDS-PAGE.
Storage	The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Storage Buffer	Tris-based buffer,50% glycerol