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Home / Proteins & Peptide

Recombinant Human TNF

SKU : BHP10900902

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Applied Biological Materials (abm) Inc.

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Mfr.No.	Z100857
Description	Tumor Necrosis Factor is secreted by macrophages, monocytes, neutrophils, T cells, natural killer cells following their stimulation by bacterial lipopolysaccharides although cells expressing CD8(+) secrete little or no TNF-alpha. In addition, TNF is secreted by peripheral neutrophilic granulocytes and by a number of transformed cell lines that include those of astrocytes, microglial cells, smooth muscle cells and fibroblasts. Human milk also contains this factor. The synthesis of TNF-alpha is induced by many different stimuli including interferons, IL2, GM-CSF, SP, Bradykinin, Immune complexes, inhibitors of cyclooxygenase and platelet activating factor. TNF-alpha shares approximately 30% homology with TNF-beta. Human TNF-alpha is a non-glycosylated protein of 17.6 kDa.
Alternative Names	Cachectin ; Differentiation-inducing factor (DIF) ; Necrosin ; Cytotoxin ; TNFSF2
Quantity	100 µg
Regulatory	RUO
Source	E. coli
Host	Human
Endotoxin Level	<1.0 EU/μg of recombinant protein as determined by the LAL method
Biological Activity Comment	The ED50 as determined by dose dependent proliferation of IL4 stimulated human megakaryocytic cell line was found to be ≤0.5 ng/mL

Weight	17.6 kDa

Description	A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Format	Lyophilized Powder Lyophilized from a 0.2 μm filtered solution in PBS (pH 7.2)
Purity	>95% as determined by SDS-PAGE
Storage	The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Immunogen Species	Homo Sapiens (Human)

MSDS	Growth Factors, Cytokines & Kinases MSDS
Other	Growth Factor and Cytokine Brochure

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SKU	Supplier No.	Size	Your price	Quantity
BHP10900902	Z100857	100µg	$129.44		Add to Cart

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