Thrombopoietin, known also as TSF (thrombopoiesis stimulating factor), for mediator substances that function as natural stimulators of megakaryocytopoiesis and thus, promote the differentiation of platelets. Thrombopoietin stimulates an increase in the sizes and numbers of megakaryocytes. It also increases the number of small acetyl-cholinesterase positive cells that are early precursor cells of the megakaryocytic lineage. Thrombopoietin is a protein of 332 amino acids that shows significant homology with Epo (erythropoietin) in its N-terminal domain. A comparison of human and porcine Thrombopoietin sequences shows 83 % identity between the erythropoietin-like domains but only 67 % between the C- terminal domains. Recombinant Human TPO expressed from human 293 cells as a monomeric glycoprotein with an apparent molecular mass of 80 to 85 kDa.
Alternative Names
THPO ; Thrombopoietin ; C-mpl ligand ; ML ; Megakaryocyte colony-stimulating factor ; Megakaryocyte growth and development factor ; MGDF ; Myeloproliferative leukemia virus oncogene ligand
Quantity
10 µg
Regulatory
RUO
Source
HEK293
Host
Human
Endotoxin Level
<1 EU/µg of recombinant protein as determined by the LAL method
Biological Activity Comment
The EC(50) as determined by the dose-dependent stimulation of the proliferation of MO7e cells was found to be ≤ 4 ng/mL.
Weight
80 to 85 kDa, monomer, glycosylated
Description
A quick spin of the vial followed by reconstitution in sterile 1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).
Format
Lyophilized Powder1x PBS
Purity
>95% as determined by SDS-PAGE
Storage
The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months. Avoid repeated freeze/thaw cycles.