An EGF family member, TGF-A is a cytokine that is produced following the protease digestion of its trans-membrane precursor proTGF-A. TGF-A is expressed both in normal tissues during embryogenesis and in adult tissues, such as the brain, keratinocytes, and macrophages and at high levels in transformed and tumour cells.
Reconstitute at 100 μg/mL in sterile 10 mM Acetic Acid
Formulation
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA
Molecular Weight
5.8 kDa
Quantity
20 µg
Source
E. coli
Usage
For research use only. Not for diagnostic or therapeutic use.
Accession Number
P01135
Endotoxin Level
<1.0 EU/μg of recombinant protein as determined by the LAL method.
Bioactivity
Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED(50) for this effect is typically 0.10.4 ng/mL.
Gene Id
7039
Gene Name
TGFA
Purity
>95% as determined by SDS-PAGE
Storage
The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity.Avoid repeated freeze/thaw cycles.