Superoxide Dismutase has been isolated from media conditioned by a murine marrow-derived cell line (PB6) and from the conditioned medium of cultured mouse marrow cells. It antagonizes IL-3 dependent proliferation of cells in culture and reversibly inhibits DNA synthesis of erythroid progenitor cells.
<1.0 EU/μg of recombinant protein as determined by the LAL method.
Biological Activity Comment
The activity was measured by Pyrogallic Acid method and was found to be not less than 1.0 x 10^4 IU/mg.
Weight
17.0 kDa
Description
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Format
Lyophilized PowderSOD was lyophilized from a 0.2 μm filtered PBS solution pH 7.0.
Purity
>95% as determined by SDS-PAGE
Storage
The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.