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Recombinant Human Replication protein A 70kDa DNA-binding subunit(RPA1)

SKU : BHP10500881

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CUSABIO TECHONOLOGY LLC

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Mfr.No.	CSB-EP020088HU
Alternative Names	Replication factor A protein 1 ,RF-A protein 1Single-stranded DNA-binding protein, Replication factor A protein 1 ; RF-A protein 1Single-stranded DNA-binding protein
Antigen	RPA1
Tag	N-terminal 6xHis-SUMO-tagged
Regulatory	RUO
Relevance	As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage . In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response . It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage . Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair . Plays also a role in base excision repair (BER) probably through interaction with UNG . Through RFWD3 may activate CHEK1 and play a role in replication checkpoint control. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance . As part of the alternative replication protein A complex, aRPA, binds single-stranded DNA and probably plays a role in DNA repair. Compared to the RPA2-containing, canonical RPA complex, may not support chromosomal DNA replication and cell cycle progression through S-phase. The aRPA may not promote efficient priming by DNA polymerase alpha but could support DNA synthesis by polymerase delta in presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange
Source	E.coli
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Expression Region	2-616aa
Mol Weight	84.0kDa
Nature	recombinant
Protein Description	Full Length of Mature Protein

Subcellular Location	Nucleus, Nucleus, PML body
Uniprot	P27694, P27694
Database Links	HGNC:10289 OMIM:179835 KEGG:hsa:6117 STRING:9606.ENSP00000254719 UniGene:Hs.461925
Function	As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism
Pathway	DNA repair pathway
Protein Families	Replication factor A protein 1 family
Research Area	Epigenetics and Nuclear Signaling
Weight	84kDa

Stotage Note	Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Buffer	Tris-based buffer，50% glycerol
Purity	Greater than 90% as determined by SDS-PAGE.
Storage	The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Storage Buffer	Tris-based buffer,50% glycerol

Immunogen Species	Homo Sapiens (Human)

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SKU	Supplier No.	Size	Your price	Quantity
BHP10500881	CSB-EP020088HU	1mg	$1812		Add to Cart
BHP10500881	CSB-EP020088HU	100ug	$419		Add to Cart
BHP10500881	CSB-EP020088HU	20ug	$224		Add to Cart

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