Neuregulins are EGF-like growth and differentiation factors that signal through tyrosine kinase receptors of the ErbB family. The ErbB2 and ErbB4 receptors cooperate in transmission of neuregulin-1 signals in the heart, whereas ErbB2 and ErbB3 cooperate in neural crest cells.
Alternative Names
Acetylcholine receptor-inducing activity ; ARIA ; Breast cancer cell differentiation factor p45 ; Glial growth factor ; Heregulin ; HRG ; Neu differentiation factor ; Sensory and motor neuron-derived factor
Quantity
50 µg
Regulatory
RUO
Source
E. coli
Host
Human
Endotoxin Level
<1.0 EU/μg of recombinant protein as determined by the LAL method.
Biological Activity Comment
The ED(50) was determined by the dose-dependent proliferation of human MCF-7 cells was found to be < 0.3 ng/mL, corresponding to a specific activity of 2.0 x 10^6 Units/mg.
Weight
7.0 kDa
Description
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Format
Lyophilized PowderRecombinant Neuregulin-1 was lyophilized from a 0.2 μm filtered 10mM PB, pH 7.0, containing 2% mannitol, 0.5% HSA.
Purity
>95% as determined by SDS-PAGE
Storage
The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.