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Home / Proteins & Peptide

Recombinant Human NLK Protein (His & GST Tag)(Active)

SKU : BHP10302719

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Elabscience Biotechnology Co.,Ltd

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Mfr.No.	PKSH030360
Description	A DNA sequence encoding the human NLK (Q9UBE8) (Val121-Glu527) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Alternative Names	LAK1;NLK
Reconstitution	Please refer to the printed manual for detailed information.
Background	Nemo-like kinase contains 1 protein kinase domain and belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family and MAP kinase subfamily. It also contains a TQE activation loop motif in which autophosphorylation of the threonine residue (Thr-298) is sufficient for kinase activation. As a serine/threonine-protein kinase, nemo-like kinase regulates a number of transcription factors with key roles in cell fate determination. It is a positive effector of the non-canonical Wnt signaling pathway, acting downstream of WNT5A, MAP3K7/TAK1 and HIPK2. Activation of this pathway causes binding to and phosphorylation of the histone methyltransferase SETDB1. The NLK-SETDB1 complex subsequently interacts with PPARG, leading to methylation of PPARG target promoters at histone H3K9 and transcriptional silencing. The resulting loss of PPARG target gene transcription inhibits adipogenesis and promotes osteoblastogenesis in mesenchymal stem cells (MSCs). Nemo-like kinase also is a negative regulator of the canonical Wnt/beta-catenin signaling pathway.
Reactivity	Human
Tag	N-His & GST
Formulation	Supplied as sterile 20mM Tris, 500mM NaCl, pH 8.0, 10% gly
Host	Baculovirus-Insect Cells
Accession Number	Q9UBE8
Endotoxin Level	< 1.0 EU per ug as determined by the LAL method.
Bioactivity	The specific activity was determined to be 3 nmol/min/mg using MBP as substrate.
Mol Weight	74.1 kDa
Mw(kda)	74.1 kDa

Sequence Info	Val121-Glu 527

Shipping	This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt, store it immediately at<-20°C.
Stotage Note	Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Purity	> 91 % as determined by reducing SDS-PAGE.

Product Manual	Data Sheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP10302719	PKSH030360	50ug	$717		Add to Cart

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