NEK4 or Never In Mitosis A-related kinase 4 is a serine/threonine protein kinase. Northern blot show NEK4 is expressed as 2 mRNAs of 4.0 kb and 6.8 kb in heart and several other tissues. NEK4 gene encodes an 841-amino acid polypeptide and the levels of NEK4 mRNA remain invariant throughout the cell cycle . Nek4 has been identified in a genetic screen as a mediator of response to Taxol . After Taxol treatment, NEK4 promoted microtubule outgrowth, whereas NEK4 deficiency impaired G-M arrest and decreased formation of mitotic-like asters. In contrast, NEK4 deficiency sensitized cells to vincristine, which destabilizes microtubules.
Alternative Names
NRK2 ; STK2
Tag
N-GST tag
Quantity
10 µg
Regulatory
RUO
Source
Insect cells
Host
Human
Biological Activity Comment
The specific activity of NEK4 was determined to be 15 nmol /min/mg as per activity assay protocol.
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