MIP-1-beta is one of the peptides of Macrophage Inflammatory Protein-1 that is produced by macrophages following their stimulation with bacterial endotoxins. MIP-1-beta causes local inflammatory responses, and induces superoxide production by neutrophils. The aforementioned activates of MIP-1-beta are exerted following its binding to CCR5.
Alternative Names
T-lymphocyte-secreted protein HC21 ; Lymphocyte activation gene 1 protein ; LAG-1 ; MIP-1-beta ; PAT 744 ; Protein H400 ; SIS-gamma ; Small-inducible cytokine A4 ; T-cell activation protein 2 ; ACT-2 ; MIP1B ; SCYA4 ; C-C motif chemokine 4-like ; G-26
Quantity
1.0 mg
Regulatory
RUO
Source
E. coli
Host
Human
Endotoxin Level
<1.0 EU/μg of recombinant protein as determined by the LAL method.
Biological Activity Comment
Determined by its ability to chemoattract human blood monocytes using a concentration range of 2-10 ng/mL.
Weight
8.1 kDa
Description
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Format
Lyophilized PowderLyophilized from a 0.2 μm filtered PBS solution
Purity
>95% as determined by SDS-PAGE
Storage
The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity.