Leptin is a cytokine found in the adipose tissue of animals (Leptin mRNA is restricted to the adipose tissue). Leptin has been shown to suppress appetite, increase thermogenesis and reduce body weight, food consumption and plasma glucose levels.
Alternative Names
Obese protein ; Obesity factor ; OB
Quantity
1.0 mg
Regulatory
RUO
Source
E. coli
Host
Human
Endotoxin Level
<1.0 EU/μg of recombinant protein as determined by the LAL method
Biological Activity Comment
The ED(50) was determined by the dose-dependent proliferation of human umbilical vein endothelial cells and was found to be <0.5ng/mL.
Weight
16.4 kDa
Description
Reconstitute at 1 mg/mL in sterile 20 mM Tris-HCI (pH 8.0).
Format
Lyophilized PowderLyophilized from a 0.2 μm filtered solution in Tris-HCl and NaCl.
Purity
>95% as determined by SDS-PAGE
Storage
The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity.