JavaScript is currently not supported/disabled by this browser. Please enable JavaScript for full functionality.

Call Us 1 - 866 - 986 - 9598
[email protected]

Sign Up

Login
- My Order
- Return Requests
- Track my Order

Home / Proteins & Peptide

Recombinant Human Interleukin-35/IL-35 Protein (Fc Tag)(Active)

SKU : BHP10303110

Contact us to order

Tel

+1 866.986.9598

Email

[email protected]

Get a Quote

Request More Infos

Elabscience Biotechnology Co.,Ltd

view supplier details

Order Now

Credit card payments now incur a 3% fee.

Contact us to order

Tel

+1 866.986.9598

Email

[email protected]

Get a Quote

Ask Merchant

Request More Infos

Sample Request

Mfr.No.	PKSH031988
Description	A DNA sequence encoding the human IL35 complex composed of IL27B subunit (NP_005746.2) (Met 1-Lys 229) and the mature form of human IL12 p35 subunit (P29459) (Arg 23-Ser 219) linked by a polypeptide linker was fused with the Fc region of human IgG1 at the C-terminus.
Alternative Names	CLMF;IL-12A;IL-35;Interleukin-35;NFSK;NKSF1;P35
Reconstitution	Please refer to the printed manual for detailed information.
Background	The novel Ebi3-IL-12alpha heterodimeric cytokine has been designated interleukin-35 (IL-35); is a member IL12 family cytokine produced by regulatory T cells (Treg); but not by resting or activated effector T cells (Teff). IL-35 is a heterodimeric protein composed of IL-12α (P35) and IL-27β chains; which are encoded by two separate genes called IL12A and EBI3 (Epstein-Barr-virus-induced gene 3) respectively. Ectopic expression of IL-35 confers regulatory activity on naive T cells; whereas recombinant IL-35 suppresses T-cell proliferation. It identify IL-35 as a novel inhibitory cytokine that may be specifically produced by T(reg) cells and is required for maximal suppressive activity. IL-35 has biological activity and able to expand CD4+CD25+ Treg cells; suppress the proliferation of CD4+CD25- effector cells and inhibit Th17 cell polarization. IL-35 has been shown to be constitutively expressed by regulatory T (Treg) cells CD4(+)CD25(+)Foxp3(+) and suggested to contribute to their suppressive activity. IL-35 is a crucial mediator which provokes CD4+CD25+ T cell proliferation and IL-10 generation; another well-known anti-inflammatory cytokine; along with TGFbeta cytokine. IL-35 is a cytokine can downregulate Th17 cell development and inhibit autoimmune inflammation. It inhibited the differentiation of Th17 cells in vitro. In vivo; IL-35 effectively attenuated established collagen-induced arthritis in mice; with concomitant suppression of IL-17 production but enhanced IFN-gamma synthesis. Thus; IL-35 is a novel anti-inflammatory cytokine suppressing the immune response through the expansion of regulatory T cells and suppression of Th17 cell development.
Reactivity	Human
Tag	C-Fc
Formulation	Lyophilized from sterile PBS; pH 7.4
Host	HEK293 Cells
Accession Number	Q14213&P29459
Endotoxin Level	< 1.0 EU per ug of the protein as determined by the LAL method.
Bioactivity	Measured by its ability to bind biotinylated human IL6RB-Fch in a functional ELISA.
Mol Weight	73.4 kDa
Mw(kda)	73.4 kDa

Sequence Info	Met 1-Lys 229

Shipping	This product is provided as lyophilized powder which is shipped with ice packs.
Stotage Note	Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Purity	> 85 % as determined by reducing SDS-PAGE.

Product Manual	Data Sheet

Have you used this product?

Rate it now..!!

Login to Review

SKU	Supplier No.	Size	Your price	Quantity
BHP10303110	PKSH031988	50ug	$564		Add to Cart

ebiohippo.com provides biomedical researchers with a one-stop informatic shopping experience by transforming biotechnology products’ data into a ready-to-analyze state. We collect and unify suppliers’ data on a single platform to reduce the time of searching, uncertainty, and cost of scientific experiments.

© 2024 Biohippo. All rights reserved.