IL7 is a secreted cytokine of the hemopoietin family that primarily affects early B and T cells and plays an important role in stimulating the proliferation of lymphoid progenitors. IL7 is produced by stromal epithelial cells of the thymus, bone marrow, and intestines. Human and murine IL7 share 60% sequence homology at the primary level and known to exhibit cross species activity. Designated CD127, the human IL7 receptor is a strongly glycosylated membrane protein of 76 kDa. Recombinant Human IL7 is expressed in human 293 cells as a monomeric glycoprotein, with three seperate bands of 19, 25, and 30 kD under reducing conditions on gel.
Alternative Names
IL-7
Quantity
10 µg
Regulatory
RUO
Source
HEK293
Host
Human
Endotoxin Level
<1 EU/µg of recombinant protein as determined by the LAL method
Biological Activity Comment
The EC(50) as determined by the dose-dependent stimulation of the proliferation of murine 2E8 cells was found to be ≤ 0.6 ng/mL.
Weight
19, 25, and 30 kDa, monomer, glycosylated
Description
A quick spin of the vial followed by reconstitution in sterile 1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).
Format
Lyophilized Powder1x PBS
Purity
>95% as determined by SDS-PAGE
Storage
The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months. Avoid repeated freeze/thaw cycles.