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Home / Proteins & Peptide

Recombinant Human G-CSF (CSF3)

SKU : BHP10901030

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Applied Biological Materials (abm) Inc.

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Mfr.No.	Z100247
Description	G-CSF is secreted by monocytes, macrophages, and neutrophils after cell activation. It is also produced by stromal cells, fibroblasts, endothelial cells, epithelial carcinomas, acute myeloid leukemia cells, and various tumor cell lines. The synthesis of G-CSF can be induced by bacterial endotoxins, TNF, IL1 and GM-CSF. Comparison of the primary sequence of G-CSF with those of GM-CSF and M-CSF, shows that the three factors are not related to each other. Murine and human G-CSF share a sequence homology (protein) of approximately 70% and exhibit cross reactivity. G-CSF stimulates the proliferation and differentiation of hematopoietic progenitor cells committed to the neutrophils and granulocytes lineage in a dose-dependent manner.
Alternative Names	CSF-3 ; MGI-1G ; Pluripoietin ; Human Granulocyte Colony Stimulating Factor (CSF3) ; GCSF
Quantity	50 µg
Regulatory	RUO
Source	E. coli
Host	Human
Endotoxin Level	<1.0 EU/μg of recombinant protein as determined by the LAL method
Biological Activity Comment	The ED50 as determined by the dose-dependent proliferation of neuroblastoma cell line was found to be ≤5ng/ml

Weight	19.0 kDa

Description	A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Format	Lyophilized Powder Lyophilized from a 0.2 μm filtered solution in Tris and NaCl (pH 7.5)
Purity	>95% as determined by SDS-PAGE
Storage	The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Immunogen Species	Homo Sapiens (Human)

Reference	Rincon, E et al. "A map of gene expression in neutrophil-1 like cell lines." Department of Microbiology and Molecular Genetics, University of California, Davis : (2017). DOI: https://doi.org/10.1016/j.cbpb.2017.08.004. Application: Cell culture growth factors.

MSDS	Growth Factors, Cytokines & Kinases MSDS
Other	Growth Factor and Cytokine Brochure

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SKU	Supplier No.	Size	Your price	Quantity
BHP10901030	Z100247	50µg	$144.44		Add to Cart

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